NEVBD Pesticide Resistance Monitoring Program

The Northeast Regional Center for Excellence in Vector-Borne Diseases has established a pesticide resistance monitoring network covering the Northeastern region to support public health and mosquito control agencies in detecting pesticide resistance in mosquito populations.

This program offers two primary services to our network: specimen submissions to Cornell for testing and specimen collection kits. Please scroll below to learn more about these services.

If you’re interested in receiving periodic updates about the Pesticide Resistance Program please sign up for mailing list here.

Specimen Submission to Cornell University for Testing:

If you’re interested in submitting specimens to Cornell University for resistance testing please follow the instructions below. We currently test Culex pipiens and Aedes albopictus mosquitoes.

Submitting Mosquitoes

Electronic Submission Form

Note: this form is for regular, annual resistance testing only. Please contact if you have questions about a project requiring repeated sampling throughout the season.

Consult the resources below to see the contents of each kit and guidelines to ensure that your specimens arrive at the Cornell facilities alive and viable for testing.

Aedes albopictus: Submission Kit & Guidelines Culex pipiens: Submission Kit & Guidelines

If you find that you have additional kit needs later in the season, please use the Kit Request Form below.

Kit Request Form

Rearing Guidelines and Resources

NEVBD also provides information on the collection and rearing of mosquito specimens at your own facilities.

Pesticide Resistance Testing

Testing kits are available for testing adult mosquitoes as well as testing larval mosquitoes. Please choose the option that is most appropriate for your program’s needs and follow the instructions provided.

Testing Adult Mosquito Kits

The CDC bottle bioassay is designed to determine if a particular formulation (combination of the active ingredient in the insecticide and inactive ingredients) is able to kill an insect vector, such as a mosquito, at a specific location at a given time. It can detect resistance to insecticides in mosquitoes and other insects. The technique is simple, rapid, and economical, compared with alternatives. Bioassay results can help guide the choice of insecticide used for spraying. Use the information below to order Bottle Bioassay Kits directly from the CDC for testing adult mosquitoes for pesticide resistance.

If your institution or program would like to order a bottle bioassay kit, which contains bottles, insecticide formulations, a manual, and instructional video, please download and complete the CDC Bottle Bioassay Request Form and send it to

CDC Bottle Bioassay Request Form

Larvicide Efficacy Testing Kits

Use the information below to order a Larvicide Efficacy Testing Kit directly from Cornell University for testing the field efficacy of your larvicide applications​:

Kit Request Form

Larvicide Efficacy Testing Kit and Guidelines

Our partners at PacVec have developed additional resources on larvicidal resistance testing. Please access them through their YouTube channel or the PacVec Resources Website.

Pesticide Resistance Monitoring in the Northeast Region

Visit the Pesticide Resistance StoryMap to view results of this testing program since 2019.

Pesticide Resistance Maps

NEVBD routinely engages with our regional vector control and public health partners to understand their practices and resource needs for conducting pesticide resistance monitoring within their programs.


If you have any questions about the program please contact