NEVBD

The NEVBD Pesticide Resistance Monitoring Program submission system has closed for the 2025 season.

Consult the resources below to see the contents of each mosquito collection kit and guidelines to ensure that your specimens arrive at the Cornell facilities alive and viable for testing:

• Aedes albopictus: Submission Kit & Guidelines
• Culex pipiens: Submission Kit & Guidelines
• Culiseta melanura: Submission Kit & Guidelines

If you would like to request a mosquito collection kit or larvicide efficacy kit outside of the normal submission season, please use the Kit Request Form below:

Kit Request Form

Adult Mosquito Resistance Assay Kits

If your institution or program would like to order a CDC Bottle Bioassay kit directly from the CDC, please contact USbottleassaykit@cdc.gov.

CDC Bottle Bioassay kits contain:

• Bottles
• Insecticide active ingredients
• User manual
• Instructional video

Features of the CDC Bottle Bioassay:

• Designed to determine whether a specific active ingredient is able to kill mosquitoes from a specific location at a given time.
• Technique is simple, rapid, and economical.
• Results can help guide the choice of insecticide used for spraying.

Find more information about the CDC Bottle Bioassay here

Larvicide Efficacy Testing Kits

Order a Larvicide Efficacy Testing Kit directly from Cornell University through the Kit Request Form.

Kit Request Form

Larvicide Efficacy Testing Kit and Guidelines

Our full training video playlist is available on YouTube

Northeast Vector-Borne Disease Center - Training Video Playlist

Pesticide Resistance Assay Training Videos / Probar la resistencia de los zancudos

• Adult Topical Resistance Bioassays - video available in English and español

• Larval Cup Resistance Bioassays - video available in English and español

Mosquito Rearing Training Videos / Criar zancudos

This video includes guidance for rearing Aedes aegypti, Aedes albopictus, Culex pipiens, and Culiseta melanura.

Video available in English and español

Our partners at PacVec have developed additional resources on the CDC Bottle Bioassay and larvicidal resistance testing. You can access these resources by visiting the PacVec Resources Website.

Protocols

• NEVBD Modified Insecticide Resistance Enzyme Activity Protocol

Rearing Guidelines

• Culex pipiens rearing guidelines
• Aedes albopictus rearing guidelines

Peer-Reviewed Publications

Burtis JC, Bickerton MW, Indelicato N, et al. 2022. Effectiveness of a Buffalo Turbine and A1 Mist Sprayer for the Areawide Deployment of Larvicide for Mosquito Control in an Urban Residential Setting. Journal of Medical Entomology. 59(3):903-11. DOI: 10.1093/jme/tjac017

Burtis JC, Poggi JD, Duval TB, et al. 2021. Evaluation of a Methoprene Aerial Application for the Control of Culiseta melanura (Diptera: Culicidae) in Wetland Larval Habitats. Journal of Medical Entomology. 58(6):2330-7. DOI: 10.1093/jme/tjab108

Burtis JC, Poggi JD, Payne B, et al. 2021. Susceptibility of Ixodes scapularis (Acari: Ixodidae) to Permethrin Under a Long-Term 4-Poster Deer Treatment Area on Shelter Island, NY. Journal of Medical Entomology. 58(4):1966-9. DOI: 10.1093/jme/tjab054

Burtis JC, Poggi JD, McMillan JR, et al. 2021. NEVBD Pesticide Resistance Monitoring Network: Establishing a Centralized Network to Increase Regional Capacity for Pesticide Resistance Detection and Monitoring. Journal of Medical Entomology. 58(2):787-97. DOI: 10.1093/jme/tjaa236

Lehane A, baxter L, Martin E, et. al. 2026. Insecticide resistance across the Northeast and Mid-Atlantic United States: results from 6 years of monitoring (2019-2024). Journal of Medical Entomology. 63(1):tjaf171. DOI: 10.1093/jme/tjaf171

Program Reports

Poggi J, Burtis J, Mader E, Harrington L. 2020. 2019 Pesticide Use and Resistance Monitoring in the Northeastern United States. Northeast Regional Center for Excellence in Vector-Borne Diseases. Report. Available at: https://hdl.handle.net/1813/69865