NEVBD Pesticide Resistance Monitoring Program
The Northeast Regional Center for Excellence in Vector-Borne Diseases has established a pesticide resistance monitoring network covering the Northeastern region to support public health and mosquito control agencies in detecting pesticide resistance in mosquito populations.
This program offers services to our network including specimen testing at Cornell University and training resources. Please scroll below to learn more about these services.
The Pesticide Resistance Monitoring Program closed on Thursday, August 28th for the 2025 summer season.
If you’re interested in receiving periodic updates about the Pesticide Resistance Monitoring Program please sign up for our mailing list here.
Submitting Mosquitoes
The NEVBD Pesticide Resistance Monitoring Program submission system has closed for the 2025 season.
Consult the resources below to see the contents of each mosquito collection kit and guidelines to ensure that your specimens arrive at the Cornell facilities alive and viable for testing:
Aedes albopictus: Submission Kit & Guidelines Culex pipiens: Submission Kit & Guidelines Culiseta melanura: Submission Kit & Guidelines
If you would like to request a mosquito collection kit or larvicide efficacy kit outside of the normal submission season, please use the Kit Request Form below:
Training Documents and Videos
NEVBD-TEC Mosquito Rearing & Pesticide Resistance Assay Training Videos
The NEVBD-TEC has developed training videos to help mosquito control professionals learn how to perform in-house rearing and resistance assays.
- Rearing guidelines include Aedes aegypti, Aedes albopictus, Culex pipiens, and Culiseta melanura.
- Pesticide resistance assay protocols include larval cup resistance bioassays and adult topical resistance bioassays.
Rearing & Resistance Assay Training Videos
NEVBD Guidelines for Collection & In-house Rearing of Mosquito Specimens
Pesticide Resistance Testing Kits
Pesticide resistance assay kits are available for both adult and larval mosquito testing. Please choose the option that is most appropriate for your program’s needs and follow the instructions provided.
Adult Mosquito Resistance Assay Kits
The CDC bottle bioassay is designed to determine whether a specific active ingredient is able to kill mosquitoes from a specific location at a given time. The technique is simple, rapid, and economical. Bioassay results can help guide the choice of insecticide used for spraying. Use the information below to order Bottle Bioassay Kits directly from the CDC for testing adult mosquitoes for pesticide resistance.
If your institution or program would like to order a bottle bioassay kit, which contains bottles, insecticide active ingredients, a manual, and an instructional video, please contact USbottleassaykit@cdc.gov.
Larvicide Efficacy Testing Kits
Use the information below to order a Larvicide Efficacy Testing Kit directly from Cornell University for testing the field efficacy of your larvicide applications:
Larvicide Efficacy Testing Kit and Guidelines
Our partners at PacVec have developed additional resources on larvicidal resistance testing. Please access them through their YouTube channel or the PacVec Resources Website.
Pesticide Resistance Monitoring in the Northeast Region
Visit the Pesticide Resistance StoryMap to view results of this testing program since 2019.
NEVBD routinely engages with our regional vector control and public health partners to understand their practices and resource needs for conducting pesticide resistance monitoring within their programs.
Questions?
If you have any questions about the program please contact pesticide@cornell.edu
