NEVBD

The Pesticide Resistance Monitoring Program specimen submission system will reopen in summer 2025. Please subscribe to our mailing list to receive updates about the 2025 season.

Consult the resources below to see the contents of each mosquito collection kit and guidelines to ensure that your specimens arrive at the Cornell facilities alive and viable for testing.

Aedes albopictus: Submission Kit & Guidelines Culex pipiens: Submission Kit & Guidelines Culiseta melanura: Submission Kit & Guidelines

If you would like to request a mosquito collection kit or larvicide efficacy kit outside of the normal submission season, please use the Kit Request Form below.

Kit Request Form

NEVBD provides information on the collection and rearing of mosquito specimens.

• Culex pipiens rearing guidelines
• Aedes albopictus rearing guidelines

NEVBD-TEC Mosquito Rearing & Pesticide Resistance Assay Training Videos

The NEVBD-TEC has developed training videos to help mosquito control professionals learn how to perform in-house rearing and resistance assays.

• Rearing guidelines include Aedes aegypti, Aedes albopictus, Culex pipiens, and Culiseta melanura.
• Pesticide resistance assay protocols include larval cup resistance bioassays and adult topical resistance bioassays.

Rearing & Resistance Assay Training Videos

The CDC bottle bioassay is designed to determine if a particular formulation (combination of the active ingredient in the insecticide and inactive ingredients) is able to kill an insect vector, such as a mosquito, at a specific location at a given time. It can detect resistance to insecticides in mosquitoes and other insects. The technique is simple, rapid, and economical, compared with alternatives. Bioassay results can help guide the choice of insecticide used for spraying. Use the information below to order Bottle Bioassay Kits directly from the CDC for testing adult mosquitoes for pesticide resistance.

If your institution or program would like to order a bottle bioassay kit, which contains bottles, insecticide formulations, a manual, and instructional video, please download and complete the CDC Bottle Bioassay Request Form and send it to usbottleassaykit@cdc.gov.

CDC Bottle Bioassay Request Form

Use the information below to order a Larvicide Efficacy Testing Kit directly from Cornell University for testing the field efficacy of your larvicide applications​:

Kit Request Form

Larvicide Efficacy Testing Kit and Guidelines

Our partners at PacVec have developed additional resources on larvicidal resistance testing. Please access them through their YouTube channel or the PacVec Resources Website.