NEVBD Pesticide Resistance Monitoring Program

The Northeast Regional Center for Excellence in Vector-Borne Diseases has established a pesticide resistance monitoring network covering the Northeastern region to support public health and mosquito control agencies in detecting pesticide resistance in mosquito populations.

This program offers two primary services to our network: specimen submissions to Cornell for testing and specimen collection kits. Please scroll below to learn more about these services.

If you’re interested in receiving periodic updates about the Pesticide Resistance Program please sign up for mailing list here.

Insecticide Resistance in Mosquitoes: Practical Guidance & Tips for Performing Your Own Monitoring Assays

NEVBD is hosting a free webinar on conducting insecticide resistance monitoring assays for mosquitoes in the Northeast. Dr. James Burtis of Cornell University will be joined by John Shepard of the Connecticut Agricultural Experiment Station in a discussion of how resistance bioassay diagnostics are created, determining the resistance status of field-collected mosquitoes, and tips for collection mosquitoes from the field and rearing them in the laboratory setting. This webinar will be held Wednesday April 1, 2020, from 11am - 12:30pm EST.

Register Now!

2020 Pestcide Use and Resistance Survey

NEVBD is currently in the process of updating materials for our Pesticide Resistance Monitoring Network for the 2020 season. We are collecting feedback from our stakeholders to determine how to continue serving the vector control community in our region through an online survey. We are asking those working in state- and county-level public health and vector control agencies in the region to complete this survey. Your participation is essential to the continuing development of the pesticide resistance monitoring program. The survey asks questions relevant to the following topics:

  • Which state and county agencies are responsible for conducting vector surveillance and control?
  • What mosquito species are being targeted for control?
  • What pesticide products are being used in our region?
  • Did you participate in our program last season, and how can we improve?
  • Are pesticide resistance monitoring programs already established in the region, and what methods do they use?
  • What can NEVBD do to ensure our pesticide resistance monitoring program and specimen submission system are well-focused on the needs of public health practitioners throughout the Northeast?

Responding to the Pesticide Use and Resistance Survey should only take between 10 to 15 minutes to complete.

Complete the Survey

Specimen Submission to Cornell University for Testing:

If you’re interested in submitting specimens to Cornell University for resistance testing please follow the instructions below. We currently test Culex pipiens and Aedes albopictus mosquitoes.

Submitting Mosquitoes

We have developed guidelines to ensure that your specimens arrive at the Cornell facilities alive and viable for testing. Please select the kit and guidelines for your species of interest.

How to submit mosquitoes you have already collected to the Cornell testing program:

  1. Read the Specimen Submission Kit form to decide if you need to order the submission kit, or if you can send your specimens using your own supplies.
  2. If supplies are needed, order the Specimen Submission Kit using the Kit Request Form below.
  3. Complete a Specimen Submission Form, following the instructions below.

Kit Request Form Aedes albopictus: Submission Kit & Guidelines Culex pipiens: Submission Kit & Guidelines

Ready to submit your specimens?

You must fill out the “Specimen Submission Form” before sending specimens to Cornell University for pesticide resistance testing. Once the form is completed please:

  1. Send one copy to James Burtis at
  2. Include a second copy in the package that you ship.

Specimen Submission Form

Rearing Guidelines and Resources

NEVBD also provides information on the collection and rearing of mosquito specimens at your own facilities.

  • Culex pipiens rearing guidelines (coming soon)
  • Aedes albopictus rearing guidelines (coming soon)

Pesticide Resistance Testing

Testing kits are available for testing adult mosquitoes as well as testing larval mosquitoes. Please choose the option that is most appropriate for your program’s needs and follow the instructions provided.

Testing Adult Mosquito Kits

The CDC bottle bioassay is designed to determine if a particular formulation (combination of the active ingredient in the insecticide and inactive ingredients) is able to kill an insect vector, such as a mosquito, at a specific location at a given time. It can detect resistance to insecticides in mosquitoes and other insects. The technique is simple, rapid, and economical, compared with alternatives. Bioassay results can help guide the choice of insecticide used for spraying. Use the information below to order Bottle Bioassay Kits directly from the CDC for testing adult mosquitoes for pesticide resistance.

If your institution or program would like to order a bottle bioassay kit, which contains bottles, insecticide formulations, a manual, and instructional video, please download and complete the CDC Bottle Bioassay Request Form and send it to

CDC Bottle Bioassay Request Form

Testing Larval Mosquito Kits

Use the information below to order a Larvicidal Bioassay Kit directly from Cornell University for testing larval mosquitoes for pesticide resistance​:

Kit Request Form

Larval Bioassay Kit Guidelines

Our partners at PacVec have developed additional resources on larvicidal resistance testing. Please access them through their YouTube channel or the PacVec Resources Website.


If you have any questions about the program please contact:

James Burtis, PhD
NEVBD Postdoctoral Associate
Department of Entomology, Cornell University